Ionic Strength Effects in the Aminoacylation of Valine Transfer Ribonucleic Acid
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منابع مشابه
Ionic strength effects in the aminoacylation of valine transfer ribonucleic acid.
It has been shown that the enzymic addition of valine to valine transfer ribonucleic acid (tRNA) of Escherichia co2i is exceedingly sensitive to alcohol or salt concentrations and that the rate of the reaction is predictable from the Debye-Htickel equations, if it is assumed that there are somewhat more than 4 positive charges and 4 negative charges involved in the rate-determining step. (For i...
متن کاملThe aminoacylation of transfer ribonucleic acid. Recognition of methionine by Escherichia coli methionyl-transfer ribonucleic acid synthetase.
The mechanism of the recognition of methionine by Escherichia coli methionyl-tRNA synthetase was examined by a kinetic study of the recognition of methionine analogues in the ATP-PPi exchange reaction and the tRNA-aminoacylation reaction. The results show that the recognition mechanism consists of three parts: (1) the recognition of the size, shape and chemical nature of the amino acid side cha...
متن کاملStructure and function of transfer ribonucleic acid. 3. Some properties of a complex between valyl transfer ribonucleic acid synthetase and transfer ribonucleic acid specific for valine.
The formation and some properties of a stable complex between valyl transfer ribonucleic acid synthetase and transfer RNA from yeast have been investigated by three different methods for the isolation of the complex: titration over Sephadex, sucrose gradient centrifugation, and electrophoresis on Pevikon as supporting medium. Transfer RNA specific for valine (tRNAvsl) can be separated into two ...
متن کاملThe aminoacylation of transfer ribonucleic acid. Inhibitory effects of some amino acid analogues with altered side chains.
Several amino acid analogues that are able to replace amino acid residues in binding positions of the biologically active C-terminal tetrapeptide amide sequence, Trp-Met-Asp-PheNH2, of the gastrins were examined for their ability to inhibit the aminoacylation of tRNA in an Escherichia coli and rat liver system. Although in both systems the amino acid side chains are involved in the recognition ...
متن کاملStructure and function of transfer ribonucleic acid. IV. Complexes between valyl transfer ribonucleic acid synthetase and structurally modified transfer ribonucleic acid specific for valine.
The formation of a stable complex between valyl transfer ribonucleic acid synthetase from yeast and transfer RNA specific for valine (tRNAVa’) from the same source has been used as a model for the recognition between enzyme and tRNA. With the use of sucrose gradient centrifugation to isolate the complex, the major fraction of tRNAVal (tRNAp’) was found to retain its ability to form a stable com...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99435-4